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The 'Born This Way' singer and the 'Sex and the City' actress star alongside Minnie Mouse in 'Electric Holiday', which follows the cartoon character on her way to Paris Fashion Week.
Also appearing in cartoon form for the animation are Lanvin creative director Alber Elbaz, and former French Vogue editor Carine Roitfeld, among many more of the industry's faces.
The cartoon film will be broadcast in Barney's New York department store from November 14, and includes some of the most popular Disney characters strutting their stuff on the catwalk after a fashion makeover.
For their show, Minnie is dressed in Lanvin while Daisy Duck has a bespoke Dolce & Gabbana design and Cruella De Vil gets dressed head-to-toe in Rik Owens.
Nicolas Ghesquiere preps Mickey Mouse, who wears Balenciaga, Goofy turns up in Balmain, Princess Tiana wears Proenza Schouler; and Snow White is dressed by Nina Ricci.
Gaga and Sarah both have front row seats and are joined by Naomi Campbell and a number of fashion magazine editors for the show.
Speaking of the project, Mark Lee, Barneys CEO explained: "Disney came up on a very short list of dreams - thinking about if we could do anything, what would we do?
"They represent magic and Barneys New York holiday needs to be part magic and part surprise."